Nov 24, 2022

Public workspaceCell treatments for time-lapse experiments

DOI
dx.doi.org/10.17504/protocols.io.j8nlkw8bxl5r/v1
  • 1Department of Health Sciences, University of Milan, Milan, Italy
Electra Brunialti
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DOI: dx.doi.org/10.17504/protocols.io.j8nlkw8bxl5r/v1
External link: https://doi.org/10.3390/cells12030343
Protocol Citation: Electra Brunialti, Alessandro Maria Villa, Paolo Ciana 2022. Cell treatments for time-lapse experiments. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkw8bxl5r/v1
Manuscript citation:
Brunialti E, Villa A, Toffoli M, Pozo SLD, Rizzi N, Meda C, Maggi A, Schapira AHV, Ciana P, Sex-Specific Microglial Responses to Glucocerebrosidase Inhibition: Relevance to GBA1-Linked Parkinson’s Disease. Cells 12(3). doi: 10.3390/cells12030343
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 23, 2022
Last Modified: November 24, 2022
Protocol Integer ID: 73173
Funders Acknowledgement:
The work was supported by the EU Joint Programme - Neurodegenerative Disease Research (JPND) project GBA-PaCTS, 01ED2005B
Abstract
Cell treatments for time-lapse experiments.
For the timelapse experiments, cell cultures were treated for 6 h with vehicle (water) or lipopolysaccharide (LPS) at the final concentration of 10 µg/ml LPS O111:B4, (Cat. L2630, Merk) starting from a 0,5 mg/ml stock solution, and then subjected to timelapse microscopy for 2h. For the GCase inhibition, cells were treated for 48 h with 200 µM CBE (Cat. 234599, Merk) starting from a 10 mM stock solution, or vehicle (water), and then subjected to timelapse microscopy for 2h.