Aug 31, 2023
Cell Culture Reagents and Sources – 2023-08-30
- 1Department of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University, Auburn, AL 36849 USA;
- 2O'Neal Comprehensive Cancer Center, University of Alabama-Birmingham, Birmingham, AL 35233 USA
Protocol Citation: David J Riese II, Vipasha Dwivedi 2023. Cell Culture Reagents and Sources – 2023-08-30. protocols.io https://dx.doi.org/10.17504/protocols.io.261ged55wv47/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 30, 2023
Last Modified: August 31, 2023
Protocol Integer ID: 87154
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Abstract
The COVID-19 pandemic and its aftermath have created additional challenges in maintaining the supply chain to support biomedical research laboratory operations. A related challenge is recording and sharing cell culture conditions and reagents among laboratory members. Through this protocols.io publication, we attempt to address both of these challenges.
The COVID-19 pandemic and its aftermath have created additional challenges in maintaining the supply chain to support biomedical research laboratory operations. A related challenge is recording and sharing cell culture conditions and reagents among laboratory members. Through this protocols.io publication, we attempt to address both of these challenges. The spreadsheet associated with this publication 
Cell Culture Media and Selectable Markers.V5.xlsx16KB describes the media, sera, and other supplements used for propagating the cell lines in our laboratory. The spreadsheet includes the concentration of G418, hygromycin, blasticidin, and puromycin used for selecting stable recombinant derivatives of these cell lines. Finally, the spreadsheet contains the current vendor and catalog number for these cell culture reagents.
Cell Culture Media and Selectable Markers.V5.xlsx16KB describes the media, sera, and other supplements used for propagating the cell lines in our laboratory. The spreadsheet includes the concentration of G418, hygromycin, blasticidin, and puromycin used for selecting stable recombinant derivatives of these cell lines. Finally, the spreadsheet contains the current vendor and catalog number for these cell culture reagents.This manuscript will be revised and republished as we change our standard operating procedures. To assist readers in identifying the current version of this manuscript, we will include the publication date in the title.