RAW264.7 cells are a macrophage-like cell line that was derived from a male BALB/c mouse ascites tumor induced by Abelson Leukemia Virus (A-MuLV) [1]. Due to its rapid proliferation and ease of handling, the RAW264.7 cell line is widely used to study immune function, inflammation, and cancer biology. The cells have a doubling time of about 11 to 30 hours. The growth media should be refreshed every 2 to 3 days but not exceeding 4 days. RAW264.7 cells can perform phagocytosis and pinocytosis, which are essential functions of macrophages. In addition, RAW264.7 M0 macrophages can differentiate into M1 or M2 macrophages upon respective stimuli. Hence, stimulated RAW264.7 cells have also been widely used as an in vitro inflammatory model for screening the effectivity of anti-inflammatory compounds and the investigation of the underlying molecular mechanism.