A | B | |
Determined cell number of suspension | 4 x 10^6 cells per ml | |
Total volume for seeding | 5 ml | |
Required cell number for seeding | 3 x 10^6 cells in 5 ml | |
Calculation: | ||
Required cell suspension | 0.75 ml | |
Fresh high-glucose DMEM (containing 0.1 mg/ml PSG) to add | 4.25 ml |
A | B | C | D | E | F | G | |
25 cm2-flask | 6-well plate | 12-well plate | 24-well plate | 48-well plate | 96-well plate | ||
Cell number/flask or well | 3 x 10^6 | 1 x 10^6 | 0.5 x 10^6 | 0.25 x 10^6 | 0.1 x 10^6 | 0.05 x 10^6 | |
Volume/flask or well | 5 ml | 2 ml | 1 ml | 0.5 ml | 0.3 ml | 0.2 ml |