Aug 22, 2023

Public workspaceCAMbank: SST Field Processing v1

DOI
dx.doi.org/10.17504/protocols.io.81wgbxxo1lpk/v1
  • 1Weill Cornell Medicine;
  • 2BioAstra
Eliah G Overbey
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.81wgbxxo1lpk/v1
Protocol Citation: Eliah G Overbey, Krista A Ryon, JangKeun Kim, Christopher E Mason 2023. CAMbank: SST Field Processing v1. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxxo1lpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 22, 2023
Last Modified: June 27, 2024
Protocol Integer ID: 86809
Keywords: CAMbank, SST, serum, astronaut, SOMA
Funders Acknowledgement:
WorldQuant Foundation
NASA
Grant ID: NNX14AH50G
NASA
Grant ID: NNX17AB26G
NASA
Grant ID: 80NSSC22K0254
NASA
Grant ID: NNH18ZTT001N-FG2
NASA
Grant ID: 80NSSC22K0254
NASA
Grant ID: NNX16AO69A
NASA
Grant ID: 80NSSC21K0316
Abstract
Field processing of SST vacutainers for the Cornell Aerospace Medicine Biobank (CAMbank).

Instructions for preserving: Serum and RBC Pellets.
Materials
Tube Type: BD Vacutainer® Serum Separator Tubes (SST) (BD Biosciences: #367987)
Perform Venipuncture
Perform Venipuncture
5m
5m
After venipuncture, invert the tubes gently 8 to 10 times to fully mix tube anticoagulant with blood sample.

Store the tube upright at room temperature until centrifugation.

Allow the blood to clot in an upright position for at least 30 minutes.
For optimal biochemical results, centrifuge and aliquot SSTs within 3 hours of venipuncture.
5m
Centrifuge Settings
Centrifuge Settings
15m
15m
Note: A swing bucket centrifuge is required.

Set centrifuge:
  • acceleration: 9
  • deceleration: 9
  • temperature: RT
  • duration: 10 minutes
  • speed: 1300xg
3m
Place the SSTs in the centrifuge.
Place a protective cover over the swing buckets in case of tube leakage.
Start the centrifuge.
Stand by the centrifuge until the centrifuge reaches max speed. Listen for signs of imbalance or compromised tube integrity.
12m
Aliquot Serum
Aliquot Serum
22m
22m
Carefully remove SSTs from the centrifuge and inspect for separation of red blood vs serum layers.

Transfer SSTs to a sanitized laminar flow hood.
2m
Using a P1000, carefully aliquot serum into 2D barcoded tubes at 500µL each.

Return 2D tubes with serum to the rack and place in the -80C freezer.
20m
Recap and freeze the SST tubes to preserve DNA in the red blood cell pellet