1. Adaptor-ligated, size-selected, indexed double-stranded DNA libraries
- NGS sheared DNA or libraries: The total DNA in the samples must be between 100 pg/μL to 10 ng/μL.
- PCR samples: The total DNA in the sample must be between 5 – 500 pg/μL.
2. For Agilent High Sensitivity DNA Assay (Bioanalyzer 2100):
2a. Agilent High Sensitivity DNA Kit (Agilent, Cat no. 5067-4626)
- For reorder: Agilent High Sensitivity DNA Reagents (Agilent, Cat no. 5067-4627)
2b. Agilent 2100 Bioanalyzer System incl. Chip Priming Station (Agilent, Cat no. 5065-4401) and IKA vortex mixer
2c. Single-channel pipettes (P2, P10, P200 and P1000) with compatible, sterile tips
2d. 0.5 mL low-bind microcentrifuge tubes
2e. Microcentrifuge (with speeds of >13000g)
3. For Agilent D1000 ScreenTape Assay (Tapestation 4150/4200):
3a. D1000 ScreenTape (Agilent, Cat no. 5067-5582)
3b. D1000 Reagents (Agilent, Cat no. 5067-5583)
- For reorder: D1000 Sample Buffer (5067-5602), D1000 Ladder (5067-5586)
3c. Loading tips (Agilent, Cat no. 5067-5598, 1pk or 5067-5599, 10pk)
3d. 96-well Plates (Cat no. 5042-8502 or equivalent) and 96-well Plate Foil Seal (Cat no. 5067-5154 or equivalent)
Optical Tube 8x Strip (Cat no. 401428) and Optical Cap 8x Strip (Cat no. 401425)
3e. Vortex mixer IKA MS3 with adapter
3f. Single-channel pipettes (P2, P10, P200 and P1000) with compatible, sterile tips
4. For New England BioLabs NEBNext Library Quant Assay:
4a. NEBNext® Library Quant Kit for Illumina® (NEB, Cat no. E7630)
4d. Compatible qPCR plates and seals
4e. PCR strip tubes or microcentrifuge tubes
4f. Conical centrifuge tubes