1. Double-stranded genomic DNA (quantified using Qubit and purity-checked with Nanodrop)
2. NEBNext®Ultra™II FS DNA Library Prep for Illumina w/ Sample Purification Beads (New England BioLabs, Cat no. E6177L)
Required Materials that are not included in (2):
3. 80% Ethanol (freshly prepared solution)
5. 96-well PCR-plate, low-profile, full skirted (ThermoFisher Scientific, Cat no. AB0800)
7. Magnetic rack/stand (ThermoFisher Scientific DynaMag™-96 Side Skirted Magnet, Cat no. 12027 or Alpaqua Catalyst™ 96 Slotted Ring Magnet Plate, Cat no. A000550)
11. 2.0 mL microcentrifuge tubes
13. Single- and multi-channel pipettes (P2, P10, P200, P1000) with compatible, sterile filtered pipette tips