Jan 17, 2025

Public workspaceBrain-wide delivery of Janelia Fluor HaloTag ligand dyes for tracking protein turnover in mice V.2

DOI
dx.doi.org/10.17504/protocols.io.kqdg357k1v25/v2
  • 1HHMI Janelia Research Campus
Boaz Mohar
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DOI: dx.doi.org/10.17504/protocols.io.kqdg357k1v25/v2
Collection Citation: Boaz Mohar 2025. Brain-wide delivery of Janelia Fluor HaloTag ligand dyes for tracking protein turnover in mice. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg357k1v25/v2Version created by Boaz Mohar
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 22, 2024
Last Modified: January 20, 2025
Collection Integer ID: 95630
Funders Acknowledgements:
HHMI
Abstract
These protocols are part paper titled:
DELTA: A method for brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning

Files
Protocol
Icon representing the file Preparation of JF dye for retro-orbital injection in mice
Name
Preparation of JF dye for retro-orbital injection in mice
Version 2
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Retro-orbital injection of virus or dye in mice
Name
Retro-orbital injection of virus or dye in mice
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Protocol for mouse perfusion with dye, DAPI staining, and slide preparation
Name
ForkProtocol for mouse perfusion with dye, DAPI staining, and slide preparation
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file Mesuring JF dye kinetics in the brain of C57/Bl mice
Name
Mesuring JF dye kinetics in the brain of C57/Bl mice
Version 1
, HHMI Janelia Research Campus
Boaz MoharHHMI Janelia Research Campus
Protocol
Icon representing the file 4% Paraformaldehye in .1M PB preparation
Name
4% Paraformaldehye in .1M PB preparation
Version 1
, HHMI Janelia Research Campus
Monique CopelandHHMI Janelia Research Campus