A | B | C | |
Component | Stock | Volumes for 50 ml | |
100 mM Tris-HCl | 1 M pH 8 | 5 ml | |
1M LiCl | 8 M | 6.25 ml | |
10 mM EDTA | 500 mM pH 8 | 1 ml | |
1% SDS (or LiDS) | 5% w/v | 10 ml | |
5 mM DTT | 0.5 M | 500 μl | |
Antifoam A | 750 ul | ||
RNAse-free H2O | Make up to 50 ml |
A | B | C | |
Component | Stock | Volumes for 50 ml | |
10 mM Tris-HCl | 1 M pH 8 | 500 μl | |
150 mM LiCl | 8 M | 940 μl | |
1 mM EDTA | 500 mM pH 8 | 100 μl | |
0.1% SDS | 5% w/v | 500 μl | |
RNAse-free H2O | Make up to 50 ml |
A | B | C | |
Component | Stock | Volume for 50 ml | |
10 mM Tris-HCl | 1 M pH 8 | 500 μl | |
150 mM LiCl | 8 M | 940 μl | |
1 mM EDTA | 500 mM pH 8 | 100 μl | |
RNAse-free H2O | Make up to 50 ml |
A | B | C | |
Component | Stock | Volume for 50 ml | |
10 mM Tris-HCl | 1 M pH 8 | 500 μl | |
150 mM NaCl | 5 M | 1.5 ml | |
1 mM EDTA | 500 mM pH 8 | 100 μl | |
RNAse-free H2O | Make up to 50 ml |
A | B | C | |
Component | Stock | Volume for 50 ml | |
10 mM Tris-HCl | 1 M pH 8 | 500 μl | |
RNAse-free H2O | Make up to 50 ml |
A | B | C | |
Component | Stock | Volume for 50 ml | |
1 M 2-Mercaptoethanol | 14.3 M | 7 μl | |
RNAse-free H2O | 93 μl |
A | B | C | |
Component | Stock | Volume for 5 ml (aimed concentration) | |
PEG 8000 | 1.5 ml (15%) | ||
NaCl | 2.5 ml (2.5 M) | ||
RNAse-free H2O | 1 ml |
A | B | C | |
Component | Stock conc. | Volume for 1 mL | |
Tris-HCl | 10 mM | 999 μl | |
2-Mercaptoethanol | 1 M | 1 μl |
A | B | |
Component | Volume | |
RDD buffer | 1.85 uL | |
DNAase I | 0.46 uL | |
total | 2.31 uL |
A | B | |
Component | Volume | |
5X Thermo Scientific RT buffer | 1.5 μl | |
3-prime priming adapter | 1 μl |
A | B | |
Component | vol/rxn | |
5X Thermo Scientific RT buffer | 1.5 μl | |
0.5M DTT | 0.3 μl | |
H2O | 2.2 μl | |
25mM dNTPs | 0 .5 μl | |
RevertAid RT enzyme | 0.5 μl |
A | B | |
Component | Volume | |
50 mM EDTA pH 8.0 | 5 μl | |
Ampure beads | 30 μl |
A | B | |
Component | Volume | |
H2O | 3.5 μl | |
10X PolI buffer | 1 μl | |
250 mM MgCl2 | 1 μl | |
25 mM dNTPs | 0 .25 μl | |
DNA Pol I | 0 .25 μl |
A | B | |
Component | Volume | |
50 mM EDTA pH 8.0 | 10 μl | |
Ampure Bead Resuspension Buffer (ABR) | 30 μl |
A | B | |
Component | Volume | |
2 X KAPA | 10 μl | |
2 μM PE1 primer | 1 μl | |
8 μM each EnrichS1 + S2 primers | 1 μl | |
25mM dNTPs | 0.2 μl |