May 06, 2024

Public workspaceBovine necropsy

DOI
dx.doi.org/10.17504/protocols.io.n2bvj344wlk5/v1
Bovine necropsy
  • Laetitia Dorso1
  • 1Oniris VetAgroBio Nantes National College
  • Laetitia Dorso: EBVS® European Specialist in Veterinary Pathology
Aurélien Capitan
Open access
DOI: dx.doi.org/10.17504/protocols.io.n2bvj344wlk5/v1
Protocol Citation: Laetitia Dorso 2024. Bovine necropsy. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj344wlk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 09, 2024
Last Modified: May 06, 2024
Protocol Integer ID: 94963
Keywords: necropsy, autopsy, bovine, cattle, veterinary investigation, cause of death, animal, body examination
Abstract
A necropsy is the final medical diagnostic procedure performed on a deceased animal to determine the cause of death.
The aim of a necropsy is to investigate and define as precisely as possible the cause of death of the animal, or to identify and confirm the suspected pathological process.

It is a medical examination. It consists of several stages, and must be carried out in premises dedicated to this activity, in accordance with the regulations.
A summary report must be drawn up and signed by the pathologist to record the observations made. The samples and analyses, if needed, must be taken as soon as possible after the body'arrival at the laboratory.
Anamnesis
Anamnesis
Breeding:
Rearing method, number and age of animals
Feed
Breeding health plan, vaccines, deworming, others (nature, dates)
Morbidity, mortality (diagnosis, number and %)
Animal:
Date of introduction in the herd
Symptoms (description, chronology, duration)
Conditions, date and time of death
Treatments (nature, date)
Complementary examinations (nature, dates, results)
Necropsy technique for an adult cattle
Necropsy technique for an adult cattle
The animal is suspended by one hind leg.
Check the animal's identity (Conforming buckle)
Assessment of the condition of the animal
  • Observation of the skin (erosion, etc.), overall silhouette (swelling, oedema, amyotrophy, etc.)
  • Observation of mucous membranes (eyes, mouth) colour changes (congestion, pallor, cyanosis, petechiae)
  • Observation of the eyes and oral cavity
  • Observation of the hindquarters and rectal and vaginal mucosa looking for signs of diarrhoea, congestion and petechiae.
A T-shaped incision is made: the two horizontal branches of the T follow an axis from the caudal linea alba to the lumbar spine, and the vertical branch of the T runs from the pubic symphysis to the xiphoid process along the linea alba.
The vertical incision involves the skin, abdominal muscles and peritoneum.
  • Observation of the peritoneal cavity (presence of exudate, blood) and parietal peritoneum.
  • Identification of the body condition score based on observation of pelvic adipose tissue.
  • Section at distal rectum level.
Downward reclination of the digestive mass (using gravity) after section of all mesos along the spine.
Analysis of abdominal viscera
  • Isolate the spleen, liver and kidneys: make multiple sections in the liver parenchyma and a longitudinal section of the kidneys after decapsulation.
  • Separation of the foregut from the intestinal mass.
  • Opening of the foregut successively according to their greatest curvature (rumen, reticulum, omasum, abomasum).
  • Observe the contents of the foregut: qualitative and quantitative aspects.
  • Observe the mucosae.
Lay out the intestinal mass so as to identify the main segments (on one side duodenum, jejunum, ileum, ileocaecal valve, caecum, mesenteric lymph nodes, on the other side spiral colon, rectum).
  • Open each segment (duodenum, proximal jejunum, medial jejunum, distal jejunum, ileum, caecum, spiral colon and rectum) to a length of approximately 10 cm and observe the contents (qualitative/quantitative) and the appearance of the mucosa.
  • Open the uterus: observe the contents and mucosa.
  • Open the bladder: observe the contents and mucosa.
Analysis of the thoracic viscera:
  • Cut the diaphragm where it attaches to the ribs and check for the presence of a pleural vacuum.
  • Observe the pleural cavity (presence of exudate, blood) and the parietal pleura.
  • Cut the oesophagus and trachea cranially to remove the heart/lung block.
  • Separate the heart from the lungs.
  • Open the oesophagus longitudinally: analyse any contents and the oesophageal mucosa
  • Open the trachea to the tracheobronchial junction: analyse any contents and the tracheal mucosa.
  • Palpate the lung parenchyma carefully (cranially first, then caudally), looking for any change in consistency.
  • Make a section in each of the lobes and observe: the airways, the vessels, the alveolar parenchyma.
  • Cutting the heart: the pericardium is incised and the heart is removed. A first apical section is made about 1/3 of the apex.
  • The ventricular walls are examined and their thickness assessed. The right heart is placed on the left and the left heart on the right.
  • A second incision is made in the left ventricle, following the septum and opening the left ventricle and aorta.
  • A third incision, parallel to the second, is made through the left ventricle and left atrium.
  • A fourth incision is made in the right ventricle, following the septum and opening the right ventricle and pulmonary artery.
  • A fifth incision, parallel to the fourth, is made through the right ventricle and right atrium.
  • Observation of heart valves and myocardium
  • Observation of lymph nodes in the carcase
  • Observation of joints and feet

Record each observation in the following sections, using the tables provided.
Identification
Identification
ABCD
Ear tagSexAgeBreed
Necropsy findings
Necropsy findings
ABCD
Body Condition Score / 5 :□ Correct□ Amyotrophy□ Leanness
ABCD
Status of preservation□ Correct□ Poor□ Autolysed
ABCDEF
Exterior appearance □ No significant lesion (NSL)□ Diarrhoea spots□ Limb swelling□ Scabs□ Other lesions
ABC
Abdominal cavity□ NSL
□ Effusion□ Hemorrhagic
□ Sero-hemorrhagic
□ Fibrinous
□ Suppurated
□ Adhesions□ Suppurated
□ Fibrosis
ABCDE
Oral cavity□ NSL□ Ulcerations□ Other
Teeth□ NSL□ Abnormal coloration□ Abnormal abrasion□ Other
Tongue□ NSL□ Ulcerations□ Other
Oesophagus□ NSL□ Ulcerations□ Obstructions□ Other
ABCDEF
RumenContent□ NSL□ Dry□ Liquid□ Other
Mucosa□ NSL□ Ulcers□ Melanosis□ Other
ReticulumContent□ NSL□ Dry□ Liquid□ Other
Mucosa□ NSL□ Ulcers□ Melanosis□ Other
OmasumContent□ NSL□ Dry□ Liquid□ Other
Mucosa□ NSL□ Ulcers□ Melanosis□ Other
AbomasumContent□ NSL□ Dry□ Liquid□ Hemorrhagic
Mucosa□ NSL□ Ulcers□ Hemorrhagic□ Fundus
□ Chronic□ Pylorus
ABCDEF
Duodenum
Content □ NSL□ Hemorrhagic□ Fibrinous□ Parasites□ Other
Mucosa□ NSL□ Congestive□ Fibrin□ Necrosis□ Other
Jejunum
Content □ NSL□ Hemorrhagic□ Fibrinous
Mucosa□ NSL□ Congestive□ Fibrin□ Necrosis□ Thickening
□ Parasites□ Other
Ileum
Content □ NSL□ Hemorrhagic□ Fibrinous□ Other
Mucosa□ NSL□ Congestive□ Fibrin□ Necrosis□ Thickening
□ Parasites□ Other
Colon
Content□ NSL□ Hemorrhagic□ Fibrinous□ Parasites□ Other
Rectum
Content □ NSL□ Hemorrhagic□ Fibrinous□ Parasites□ Other
Mucosa□ NSL□ Congestive□ Fibrin□ Necrosis□ Other
Mesenteric lymph nodes□ NSL□ Increased size□ Necrosis□ Suppuration
ABCDE
LiverSize□ NSL□ Increased□ Reduced
Shape□ NSL□ Rounded
Color□ Red□ Beige□ Orange
Consistency□ NSL □ Increased□ Reduced
□ Abscess□ Necrosis
Gall bladder□ NSL□ Repletion□ Emptiness
ABC
Pleural cavity□ NSL
□ Effusion□ Hemorrhagic
□ Sero-hemorrhagic
□ Fibrinous
□ Suppurated
□ Adherences□ Fibrinous
□ Fibrosis
ABCDEF
Nasal cavities□ NSL□ Congestion□ Fibrin□ Necrosis□ Suppuration
Trachea□ NSL□ Congestion□ Fibrin□ Ulcer□ Foam
Larynx□ NSL□ Congestion□ Fibrin□ Ulcer□ Foam
LungsSize□ NSL□ Increased□ Reduced
Shape□ NSL□ Rounded
Color□ Pink□ White
Lesion characteristics (if lung involvement)
Distribution □ Focal□ Multifocal□ Extensive□ Disseminated
Affected percentage□ 0-25%□ 25-50%□ 50-75%□ 75-100%
Demarcation□ Clear□ Blurred
Exudate□ NSL□ Foam□ Mucus□ Suppurated
Tracheobronchial lymph nodes □ NSL□ Increased size□ Necrosis□ Suppurated
ABCDE
HeartPericardium□ NSL□ Hemorrhagic
□ Sero-hemorrhagic
□ Fibrinous
□ Suppurated
Myocardium□ NSL□ Hypertrophy□ Necrosis
Valve□ NSL□ Endocarditis
Abnormalities□ Ventricular septal defect□ Atrial septal defect□ Patent ductus arteriosus
Heart.jpg


ABCDE
KidneySize□ NSL□ Increased□ Dicreased
Shape□ NSL□ Modified
Color□ Red□ Beige□ Other
Consistency□ NSL□ Increased□ Dicreased
□ Tumor infiltration□ Necrosis□ Other
Tumour infiltration
ABCDEF
JointJoint cavity□ NSL□ Hemorrhage□ Fibrin□ Suppuration
Joint cartilage□ NSL□ Erosion□ Necrosis
Bones□ NSL□ Osteomyelitis
Muscles□ NSL□ Hemorrhage□ Necrosis□ Degeneration
ABCDE
UdderColor□ Beige□ Red□ Purplish
Size□ NSL□ Increased□ Dicreased
Consistency□ NSL□ Increased□ Dicreased
□ Abscess□ Milk modification□ Necrosis
ABCDE
Genital system
Ovary / testicle□ NSL□ Other
Penis□ NSL□ Other
Uterus□ NSL□ Pregnant□ Exudate□ Hemorrhagic
□ Suppurated
□ Necrotic
ABCDE
Brain□ NSL□ Hemorrhage□ Necrosis□ Abscess
Meninges□ NSL□ Congestion□ Fibrin□ Suppuration
Assesment of lesions
Assesment of lesions
Assessment of lesions
Significant lesions
Non significant lesions
Conclusion
Analyze