Protocol Citation: HANNAH ZHU 2023. Bottom agar: 1.5% w/v LB(Miller) agar. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8pk48g2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 29, 2023
Last Modified: November 29, 2023
Protocol Integer ID: 91552
Abstract
How to make the bottom agar for plaque assay experiment
| Components | Final concentration | For 1 L | |
| Agar | 1.5% | 15 g | |
| NaCl | 1% w/v | 10 g | |
| Tryptone | 1% w/v | 10 g | |
| Yeast extract | 0.5% w/v | 5 g |
Dissolve following components in 1 L MilliQ water
Autoclave at 121 °C for 00:15:00
15m
Pour onto petri dishes (approximately 20 mL per plate (10 cm diameter plates)).
Store at 4 °C