Mar 31, 2020

Public workspaceBlunt end ligation V.1

DOI
dx.doi.org/10.17504/protocols.io.bdywi7xe
  • 1Department of basic medicine, school of medicine, tsinghua university
  • LanXun_lab
Keyong Sun
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.bdywi7xe
Protocol Citation: Keyong Sun 2020. Blunt end ligation. protocols.io https://dx.doi.org/10.17504/protocols.io.bdywi7xe
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: March 20, 2020
Last Modified: March 31, 2020
Protocol Integer ID: 34550
Materials
MATERIALS
ReagentT4 DNA Ligase - 100,000 unitsNew England BiolabsCatalog #M0202L
ReagentT4 Polynucleotide Kinase - 2,500 unitsNew England BiolabsCatalog #M0201L




Prepare DNA Fragment
Prepare DNA Fragment
Amplify target DNA fragment using high-fidelity DNA polymerase for blunt end fragment
Purify DNA using gel extraction





Phosphorylation of Recovered DNA
Phosphorylation of Recovered DNA

Ligation
Ligation