Mar 01, 2025

Public workspaceBiosynthesis of copper oxide nanoparticles with and without capping agents V.2

DOI
dx.doi.org/10.17504/protocols.io.4r3l29r6pv1y/v2
  • 1Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai
Mathurada Sasarom
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DOI: dx.doi.org/10.17504/protocols.io.4r3l29r6pv1y/v2
Protocol Citation: Siriporn Okonogi, Mathurada Sasarom 2025. Biosynthesis of copper oxide nanoparticles with and without capping agents. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l29r6pv1y/v2Version created by Mathurada Sasarom
Manuscript citation:
Influence of capping agents on physicochemical properties and leukemic cytotoxicity of copper oxide nanoparticles biosynthesized using Caesalpinia sappan extract
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 19, 2025
Last Modified: March 01, 2025
Protocol Integer ID: 123603
Keywords: metal nanoparticles, green synthesis, plant extract
Funders Acknowledgements:
Thailand Research Fund through the Research and Researcher for Industry (RRi)
Grant ID: PHD59I0076
Abstract
This protocol describes the synthesis of copper oxide nanoparticles using a Caesalpinia sappan extract as a reducing agent with and without different capping agents. Copper oxide nanoparticles were prepared by reducing copper sulfate dissolved in water with the aqueousextract of C. sappan according to our previously described method (Sasarom et al, 2023).
Materials
Materials
1. Copper sulfate pentahydrate
2. Gelatin
3. Polyethylene glycol 400
4. Polysorbate 80
5. Octyl phenol ethoxylate
6. Sodium lauryl ether sulfate
7. Mannitol
8. Gelatin
9. Lyophilized powder of the extract of C. sappan
10. Ethanol
11. Sodium hydroxide
12. Milli-Q water
Equipment
1. Magnetic stirring
2. A centrifuge
3. a sonicator
4. An oven
Biosynthesis of copper oxide nanoparticles (CuONPs)
Biosynthesis of copper oxide nanoparticles (CuONPs)
19 mL of 10 mM copper sulfate (or 44 mg per solution) were heated to 70°C for 10 min under magnetic stirring.
10 mL water, or 10 mL of studied capping agents (Gelatin, polyethylene glycol 400, polysorbate 80, octyl phenol ethoxylate, sodium lauryl ether sulfate and mannitol) was added to the copper solution.
60 mg of the lyophilized powder of the extract of C. sappan was dissolved in 6 mL water.
After 5 min of sonication, 1 mL of the extract was added to the different copper sulphate solutions which were subsequently stirred for 30 min at 70°C.
The acid mixtures (pH ~3) were adjusted to pH 10 by addition of 400 µL of a 1 M NaOH solution and stirred for 2 h at 70°C.
The excess extract was removed by centrifugation of the nanoparticle dispersion at 8000×g for 30 min.
The obtained pellet was redispersed in 30 mL milli-Q water and this precipitation/washing procedure was repeated two times.
The precipitate was dispersed in absolute ethanol and dried at 60°C for 8 h.
The particles were transferred into light-protecting containers and stored in a desiccator at room temperature until use.
Protocol references
Sasarom M, Wanachantararak P, Chaijareenont P, Okonogi S. Biosynthesis of copper oxide nanoparticles using Caesalpinia sappan extract: In vitro evaluation of antifungal and antibiofilm activities against Candida albicans. 2023;17(4):238–247.