Jun 02, 2017
Version 2
BG-11 Trace metal mix A5 V.2
- Dr. Steven Wilhelm
- The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
- CyanoHABs
Protocol Citation: Dr. Steven Wilhelm 2017. BG-11 Trace metal mix A5. protocols.io https://dx.doi.org/10.17504/protocols.io.h92b98e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: June 02, 2017
Last Modified: March 13, 2018
Protocol Integer ID: 6170
Abstract
Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.
Materials
STEP MATERIALS
Boric acidFisher ScientificCatalog #BP1681
Manganese chlorideFisher ScientificCatalog #7773-01-5
Zinc sulfate
Sodium molybdate dihydrate
Copper SulfateP212121
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Boric acidFisher ScientificCatalog #BP1681
Manganese chlorideFisher ScientificCatalog #7773-01-5
Zinc sulfate
Sodium molybdate dihydrate
Copper SulfateP212121
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Protocol materials
Copper SulfateP212121
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Zinc sulfate
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Boric acidFisher ScientificCatalog #BP1681
Sodium molybdate dihydrate
Boric acidFisher ScientificCatalog #BP1681
Manganese chlorideFisher ScientificCatalog #7773-01-5
Sodium molybdate dihydrate
Copper SulfateP212121
Manganese chlorideFisher ScientificCatalog #7773-01-5
Zinc sulfate
Boric acidFisher ScientificCatalog #BP1681
Manganese chlorideFisher ScientificCatalog #7773-01-5
Zinc sulfate
Sodium molybdate dihydrate
Copper SulfateP212121
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Add 1 L Milli-Q H2O to a clean media bottle
Add 2.86 g H3BO3
Boric acidFisher ScientificCatalog #BP1681
Add 1.81 g MnCl2*4H2O
Manganese chlorideFisher ScientificCatalog #7773-01-5
Add 0.222 g ZnSO4*7H2O
Zinc sulfate
Add 0.39 g NaMoO4*2H2O
Sodium molybdate dihydrate
Add 0.079 g CuSO4*5H2O
Copper SulfateP212121
Add 49.4 mg Co(NO3)2*6H2O
Cobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Autoclave at 121°C for 20 m