Oct 08, 2022
Behavioral tests in rodents coupled with dopamine signaling manipulations
- 1Northwestern university
Protocol Citation: Chuyu Chen, Loukia Parisiadou 2022. Behavioral tests in rodents coupled with dopamine signaling manipulations. protocols.io https://dx.doi.org/10.17504/protocols.io.261ge345jl47/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 08, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 71030
Keywords: Motor function, behavior, rota-rod, open-field, ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson’sthrough the Michael J. Fox Foundation for Parkinson’s Research (MJFF)
Grant ID: ASAP-020600
Abstract
These two protocols describe behavioral tests associated with Parkinson’s disease phenotypes in mice. The striatal motor learning protocol (rotarod test) is a two-phase paradigm assessing striatal motor learning under reversible dopamine antagonism.
Materials
Figure 1: Schematic of the rotarod training paradigm including reversal dopamine receptors antagonism.
Rotarod test
Rotarod test
General Rota-rod test is performed as follows:
The rotarod apparatus (Panlab LE8205, Harvard Apparatus) equipped with a mouse rod (3 cm diameter)
Set the rotarod with a start speed of 4 rpm, and set to 4–40 rpm acceleration over 300s
Hold the mouse by tail. Place the mouse on the rod and face away from the direction of rotation.
Start acceleration and record the time for each mouse to fall.
Perform five trials with intertrial-interval = 15 s, max trial duration = 300 s (one daily session)
Calculate the average time to fall over five trials.
Details of dopamine signaling manipulations for Rota-rod
Details of dopamine signaling manipulations for Rota-rod
The specific task consists of eighteen daily sessions divided into two phases (Figure 1).
Administer intraperitoneally dopamine receptor antagonists for sessions 1-5 30 min prior to testing prepared in 0.9% saline): cocktail of 1 mg/kg SCH23390 + 1 mg/kg eticlopride, 1 mg/kg SCH23390, or 1 mg/kg eticlopride.
Following a 72-hr break, test the mice for another thirteen sessions (drug-free recovery phase)
Open field test
Open field test
The open field arena (56 x 56 cm) is used to assess locomotor activity.
Turn on the lights in the recordingg chamber. Clean the arena with 70% ethanol and adjust the camera border to match the arena edge.
Wait for 5min to allow the ethanol to evaporate completely prior test.
A mouse is placed in the center of the arena and its ambulation activity is collected by the LimeLight software for 20 minutes.
Clean the arena with 70% ethanol between tests.
The LimeLight software provides the total distance traveled and the percentage of time/distance within different parts of the arena.