May 30, 2022
Version 1
Beetle rearing media V.1
- 1KMU
Protocol Citation: Yin-Tse Huang, Tina 2022. Beetle rearing media. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv59d3jg1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: May 23, 2022
Last Modified: September 13, 2023
Protocol Integer ID: 63053
Abstract
ambrosia beetle rearing
The recipe produces enough medium to fill approximately 36 culture tubes.(12cm)
First, add powder or solid, then add liquid
| A | B | |
| component | amount | |
| streptomycin | 0.35 g | |
| Wesson's salt mixture | 1.25 g | |
| yeast | 8 g | |
| corn starch | 8 g | |
| casein | 10 g | |
| agar | 30 g | |
| sucrose | 5 g | |
| wheat germ oil | 2.5 ml | |
| 95% ethanol | 5 ml | |
| redbay sawdust | 200 g | |
| deionized water | 580 ml |
All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.
The culture tubes was autoclaved at 121 °C for 30 min.
Put culture tubes to dry 2 night in oven.
(cotton plugs don't remove)