License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: May 23, 2022
Last Modified: September 13, 2023
Protocol Integer ID: 63053
Abstract
ambrosia beetle rearing
The recipe produces enough medium to fill approximately 36 culture tubes.(12cm)
First, add powder or solid, then add liquid
A
B
component
amount
streptomycin
0.35 g
Wesson's salt mixture
1.25 g
yeast
8 g
corn starch
8 g
casein
10 g
agar
30 g
sucrose
5 g
wheat germ oil
2.5 ml
95% ethanol
5 ml
redbay sawdust
200 g
deionized water
580 ml
All ingredients were mixed thoroughly, then put cotton plugs into the mouth of the filled tubes.
The culture tubes was autoclaved at 121 °C for 30 min.