May 22, 2024
Bedding Test
- daniel.dautan daniel1,2,
- Per Svenningsson1,2
- 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2024. Bedding Test. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj3ko5lk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 22, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95639
Keywords: ASAPCRN, behavior, fine motor skills
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Used to assess nigrostriatal sensorimotor function in mice. Based on the Deacon 2006 protocol.
Materials
Cotton nestlets
Transfer the mice as single cage into a “Green line” cage (Techniplast, USA).
Provide few pellet of food (Standard diet, SDS, R1/CRM/R3) (around 2-3 pellets for a total of 5g) as well as water ad libitum.
Place a 5 x 5 cm cellulose paper tissue folded in the right corner of the cage.
Place the cage in a ventilated rack with temperature, humidity and light control for 48h.
At the end of the 48h check the animal cage and take a picture.
Score the bedding as follow:
0. The mice did not touch the paper square.
- The mice unfolded the paper square but did not tear it up.
- The mice unfolded and started to tore up <10% of the paper square.
- The mice tore up the paper square <50%
- The mice tore up the paper square, but the bedding is not perfect.
- Perfect bedding
Protocol references
Deacon, Robert MJ. "Assessing nest building in mice." Nature protocols 1.3 (2006): 1117-1119.