Mar 01, 2022
Beam Break and Nesting
- 1University of Ottawa
Protocol Citation: Haley Geertsma 2022. Beam Break and Nesting. protocols.io https://dx.doi.org/10.17504/protocols.io.b5q2q5ye
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58874
Abstract
This protocol is used to test mice in the Beam Break and Nesting behavioural test.
Materials
Beam Break apparatus
Cotton nestlet square
Start Fusion software to begin recording and confirm the lights are on the appropriate light-dark cycle.
5m
Single-cage mice in the Beam Break rack with enough food and water for 48-hours.
1d
Turn off Fusion software and add a square of cotton nestlet. Leave mice for 17-19 hours.
5m
Record and/or score nests as per https://doi.org/10.1038/nprot.2006.170.
20m
Return mice to their home cages.
20m