Here I describe two methods for barcoding microalgae.
One starts with extracted DNA. In that case, I use Promega GoTaq, and so far, it has performed well. If I am only barcoding, I prefer using Phire Direct PCR. as it doesn't require DNA extraction.
Both methods offer the possibility of using master mixes that include loading dye, which saves time and does not affect Sanger sequencing. Additionally, I use ExoSAP-IT as a cleaning method instead of a column-based kit. I am happy with the results, as I have successfully obtained sequences of nearly 1kb.