To prepare 10 mL of the solution (70% ACN, 0.1% TFA) needed for matrices:
- obtain a 20 mL glass bottle
- add 7 mL 100% ACN, 3 mL H20, and 5 uL 100% TFA
To prepare the Calibrant:
- obtain a 1.5mL amber glass vial
- obtain a 1 uL aliquots of the TofMix (aliquots should be prepared and stored a 0.5mL eppendorf provided with the TofMix kit -- refer to data sheet)
- add 74 uL 70% ACN 0.1% TFA solution to the Eppendorf and mix well
- add that mixture to the labeled amber vial
To prepare the Matrices: ( CHCA, SA, and DHB)
- obtain a 1.5 mL amber glass vial and label it with the matrix name that you will be preparing
- obtain one aliquot of the matrix from the single-use kit.
- add 100 uL of 70% ACN 0.1 %TFA to matrix Eppendorf and use the pipette to mix well
- once mixed, and all powder is dissolved, add to the amber vial
*This is the same for all 3 matrices*