Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants originating from diverse anthropogenic sources like oil spills and combustion processes (Diamond et al., 2003; Hylland, 2006). Fluoranthene, a notable PAH, has garnered attention due to its persistence, bioaccumulation potential, and adverse effects on aquatic organisms (Behera et al., 2018). However, the influence of Fluoranthene on the chemosensory behaviour of aquatic organisms, particularly epibenthic amphipods, remains poorly understood. Specifically, the influence of environmentally relevant PAHs, like Fluoranthene, on chemosensory systems and potential alterations in ecological fitness are areas requiring further investigation. Chemosensation plays a pivotal role in various behaviours of crustaceans, including amphipods, such as food location, predator detection, mate finding, and physiological indicators (Hardege et al., 2022). We conducted a controlled laboratory experiment to address this knowledge gap using P. hawaiensis as our model organism. Employing a time-to-event (TTE) methodology, which provides a robust framework for examining the effects of environmental stressors on aquatic organism behaviour and physiology, we aimed to elucidate the impact of Fluoranthene on the chemosensory responses of P. hawaiensis and develop a protocol for TTE analysis in behavioural ecotoxicology. TTE experiments offer crucial insights into how pollutants influence the timing of specific behavioural responses, such as feeding or mating activities, which are vital for the survival and reproduction of organisms in aquatic ecosystems. Thus, this protocol outlines step-by-step procedures for conducting TTE experiments using P. hawaiensis as a model organism and Fluoranthene as the toxicant of interest.