May 14, 2024
Version 4
Artificial Cerebrospinal Fluid VIII (ACSF.VIII) V.4
- Allen Institute for Brain Science1
- 1Allen Institute
Protocol Citation: Allen Institute for Brain Science 2024. Artificial Cerebrospinal Fluid VIII (ACSF.VIII). protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmxy8ol3p/v4Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 06, 2020
Last Modified: May 14, 2024
Protocol Integer ID: 99812
Keywords: Artificial Cerebrospinal Fluid, ACSF, incubation, recording, electrophysiological, RP0228,
Abstract
This protocol describes how to make Artificial Cerebrospinal Fluid VIII (ACSF.VIII). ACSF.VIII is used for multiple applications including incubation of fresh human brain slices prior to electrophysiological recording.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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