Jul 09, 2024

Public workspaceArthrospira platensis Growth Media Stock Solution Preparation

DOI
dx.doi.org/10.17504/protocols.io.j8nlk83z1l5r/v1
  • 1Upstate Algae Laboratory Corp.
Victor vmr Rodriguez
Open access
DOI: dx.doi.org/10.17504/protocols.io.j8nlk83z1l5r/v1
Protocol Citation: Victor vmr Rodriguez 2024. Arthrospira platensis Growth Media Stock Solution Preparation. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk83z1l5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 09, 2024
Last Modified: July 09, 2024
Protocol Integer ID: 103073
Keywords: arthrospira platensis growth media, spirulina, arthrospira platensis
Abstract
The purpose of this protocol is to prepare a growth media for spirulina (Arthrospira platensis). Arthrospira platensis is a cyanobacterium species usually found in alkali environments rich with carbonate and bicarbonate concentrations. Arthrospira platensis cultures thrive in media with a pH between 9 and 10, with high bicarbonate concentrations, inorganic salts, and trace Nutrients.
Protocol materials
ReagentDistilled Water
In 2 steps
ReagentArthrospira Growth Media Bulk Salts Alkaline Algae Research SupplyCatalog #ASAp-00050
In 2 steps
ReagentF/2 mediumCatalog #MKK50L
In 2 steps
Before start
Ensure you are working in a sterile aseptic environment
Materials Needed
Materials Needed
Gather Materials
1m
500 ml media bottle
1m
magnetic stir bar
1m
Weight boat
1m
P1000 pipettor
1m
P1000 pipette tips
1m
Spatula
1m
Magnetic stir bar remover magnet
1m
Roll of labeling tape
1m
Equipment Needed
Equipment Needed
Gather Equipment
1m
UV Sanitizer
1m
Magnetic Stirrer


1m
Reagents Needed
Reagents Needed
Gather Reagents
1m
ReagentDistilled WaterContributed by users

1m
ReagentArthrospira Growth Media Bulk Salts Alkaline Algae Research SupplyCatalog #ASAp-00050

1m
ReagentF/2 mediumContributed by usersCatalog #MKK50L

1m
Methods
Methods
28m
Prepare Media
1m
Fill theAmount500 mL media bottle withAmount500 mL of ReagentDistilled WaterContributed by users

1m
Add the magnetic stir bar to the media bottle and turn on the mixer to medium
1m
Add Amount7.5 g of ReagentArthrospira Growth Media Bulk Salts Alkaline Algae Research SupplyCatalog #ASAp-00050 to the media bottle

1m
Add Amount1.0 mL of ReagentF/2 mediumContributed by usersCatalog #MKK50L to the media bottle

1m
Allow the media bottle to stir until all the salts have dissolved (You may need to move the media bottle around so that the stir bar agitates all the settled nutrient salts)
10m
Using a magnet, remove the magnetic stir bar from the media bottle
1m
Place the media bottle in UV sanitizer for 10 minutes uncapped, with cap facing up
10m
First place cap on media bottle, Remove the media bottle from the UV sanitizer
1m
Label media bottle “Spirulina Culture Media” and date
1m