You can now proceed with taking Fv/Fm measurements, or running a rapid light curve. If only Fv/Fm is desired, you can click the "Fo, Fm" button. For running a rapid light curve, go to the "light curve tab". You can adjust your PAR and exposure time for each rapid light curve step by clicking "edit" and filling in the steps appropriately. To run the light curve, hit "start". Ideally, you will want your ETR curve to hit a maximum peak and level off, or start to drop (indicative of photoinhibition) once higher PAR values are reached. If you are not generating typical curves in your ETR window, you will have to trouble shoot and adjust your settings or your light curve steps, make sure your saturation pulse is appropriate for your culture as well.