Feb 16, 2023
Apparent Diffusion Coefficient Measurement of Myelofibrosis in Mouse Tibia
- Thomas L Chenevert1,
- Thomas Chenevert1
- 1University of Michigan
Protocol Citation: Thomas L Chenevert, Thomas Chenevert 2023. Apparent Diffusion Coefficient Measurement of Myelofibrosis in Mouse Tibia. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzb1z2vx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 16, 2023
Last Modified: February 16, 2023
Protocol Integer ID: 77126
Keywords: mouse tibia, myelofibrosis model, apparent diffusion coefficient, preclinical imaging protocol, technical repeatability
Funders Acknowledgement:
National Institute of Health
Grant ID: U24CA237683
Disclaimer
The ADC PIP claims hold when:
- Scanner hardware, diffusion weighted image (DWI) data acquisition method and parameters, image reconstruction, and data-reduction procedures are equivalent (or superior) to those detailed in section III.
- Use of the same animal model and interventions to induce myelofibrosis are performed as detailed in section V.
- ADC change is assessed on an individual animal basis where each animal undergoes identical procedures on the same MRI system over longitudinal timepoints.
Abstract
The goal of this Co-Clinical Imaging Research Program (CIRP) pre-clinical imaging protocol (PIP) is to provide detailed description of key steps used to achieve a stated level of technical repeatability (precision) embodied in “Claims”, for MRI measurement of apparent diffusion coefficient (ADC) in tibia bone marrow of myelofibrosis mouse models. This pre-clinical imaging procedure document will be referred to as a “profile” and adheres to a PIP MRI template provided in: https://www.protocols.io/workspaces/pre-clinical-imaging-protocols. This profile details procedures for ADC measurement from diffusion weighted imaging (DWI) acquisition and image processing in MF mouse tibia to achieve stated performance claims. Tibia bone marrow composition in MF mouse models has gradation going from proximal to distil ends of the tibia, therefore separate claims are made for volume of interest (VOI) analysis of ADC maps for each of three distinct sections along the length of the tibia (see Figure 1):
Section 1 (proximal)º VOI (~4-5mm3) within 9mm of proximal end of tibia
Section 2 (transition)º VOI (~0.4-0.5mm3) from 10 to 12mm of proximal end of tibia
Section 3 (distil)º VOI (~0.1-0.2mm3) from 13 to 14mm of proximal end of tibia
Claim 1:A measured change in the mean ADC in Section 1 VOI of MF mouse model tibia that exceeds ±0.037µm2/ms indicates a true biological change has occurred in the tibia bone marrow with 95% confidence.
Claim 2:A measured change in the mean ADC in Section 2 VOI of MF mouse model tibia that exceeds ±0.087µm2/ms indicates a true biological change has occurred in the tibia bone marrow with 95% confidence
Claim 3:A measured change in the mean ADC in Section 3 VOI of MF mouse model tibia that exceeds ±0.030µm2/ms indicates a true biological change has occurred in the tibia bone marrow with 95% confidence
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