Feb 07, 2024

Public workspaceAnti-condensation agent

DOI
dx.doi.org/10.17504/protocols.io.e6nvwdrq2lmk/v1
  • 1Behavioural Phenomics, MRC Laboratory of Medical Sciences
John Bergqvist
Open access
DOI: dx.doi.org/10.17504/protocols.io.e6nvwdrq2lmk/v1
Protocol Citation: John Bergqvist 2024. Anti-condensation agent. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwdrq2lmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2024
Last Modified: February 07, 2024
Protocol Integer ID: 94838
Keywords: condensation, 96-well plates, 24-well plates, 6-well plates
Abstract
Preventing plate lids from fogging up by applying an anti-condensation agent. This allows longer recordings of e.g. worm behaviour.
Materials
50 mL test tube
Sterile water
99% ethanol
Triton X-100

Safety warnings
Attention
Triton X-100

Pictograms:
  • GHS05, GHS07, GHS09

Hazard statements:
  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H411 Toxic to aquatic life with long lasting effects


Ethanol

Pictograms:
  • GHS02, GHS07

Hazard statements:
  • H226 Flammable liquid and vapour.
  • H319 Causes serious eye irritation.
Before start
Make sure to treat lids that are for use at least a day after treatment.
Making anti-condensation agent
Making anti-condensation agent
Make a mixture of 0.05% Triton X-100 and 20% ethanol in a 50 mL test tube.

For 20 mL, mix:
Amount4 mL 99% ethanol
Amount0.1 mL Triton X-100
Amount16 mL sterile water
Treating plate lids
Treating plate lids
1h 0m 30s
Treating lids with the anti-condensation agent should be done at least a day before using the plates to study worm behaviour.
In a Class II Microbiological Safety Hood, pour ca. Amount3-4 mL of the anti-condensation agent on the inside of the lids and leave for Duration00:00:30 seconds .

Ensure all of the lid is covered by the anti-condensation agent.
30s
Pour the liquid back into the 50 mL tube for later use, or onto new lids to be treated.
Leave lids to dry for ca Duration01:00:00 hour .

1h
After drying, you will most likely see rings of anti-condensation agent residue on the plate. Wipe these off the lids with Kimberly-Clark Kimwipes until there are no signs of these rings.
Protocol references
Brewster JD, "A simple micro-growth assay for enumerating bacteria", J Microbiol Methods. 2003 Apr;53(1):77-86.
[https://doi.org/10.1016/S0167-7012(02)00226-9](https://doi.org/10.1016/S0167-7012(02)00226-9 "Persistent link using digital object identifier")