Transfer 100 µl of the diluted serum samples. One serum sample per well. Incubate
at room temperature (+18 0 C to +25 0 C) for 30 minutes.
Washing: Aspirate off the liquid from each well and wash 3 times each with 450µl working-
strength universal buffer on a rocking shaker. Leave the wash buffer in each well
for 30 to 60 seconds per washing cycle, and then empty the wells.
Pipette 100 µl ready for use diluted enzyme conjugate (peroxidase-labelled anti-
human-IgG) into each of the microplate wells. Incubate for 30 minutes at room
temperature (+18 0 C to +25 0 C).
Washing: Aspirate off the liquid from each well. Wash as described above.
Pipette 100µl chromogen/substrate solution into the channels of the incubation tray.
Incubate for 15 minutes at room temperature (+18 0 C to +25 0 C), protect from
Stopping: Aspirate off the liquid from each well and pipette 100µl of stop solution into of the
microplate wells in the same order and same speed as the chromogen/substrate
Evaluation: Results should be read once strips have dried.