autoclave and store at RT or 4°C
KNOP solid working solution:
top up water to 1L after adjusting pH
KNOP liquid working solution:
10 gr of sucrose (1% w/v final concentration)
40mM MES (very important)
top up water to 1L after adjusting pH
Filter sterile (do not autoclave), aliquot into 50mL falcon tubes and store at -20°C.
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Sterile disposable scalpels (#0501, Swann Morton)
Razor blades (#11904325, Fisher Scientific)
100 µm cell strainer (#352360, CORNING),
6-well plate (#140675, ThermoFisher)
3',5'-dimethoxy-4'-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA))
Cefotaxime (#BIC0111, Apollo Scientific)
Hygromycin (#10687010, Invitrogen)
Corning Disposable Vacuum Filter/Storage Systems (#430767)