A schematic for three different experimental designs that accommodate 1, 2 and 3 drugs respectively, left to right. Depicted are 4 wells of a 96 well plate that are filled with a total volume of 200uL (right). Stock concentrations of drugs are not pictured here in this generalizable model. Working dilutions from the stock include 1000x (in DMSO) and 2x (in media) for a single drug setup, 2000x (in DMSO) and 4x (in media) for a two drug setup, and 4000x (in DMSO) and 4x (in media) for a three drug setup. In all cases, 50,000 cells are delivered to each well, but in different volumes, thus requiring different starting cell concentrations. 100uL of 0.5 million cells per mL (2x the final cell concentration in a well) are required for single and two drug setups while the three drug setup requires 50uL of 1 million per mL cells (4x the final concentration in a well). Unique to the three drug setup is an addition of 1uL to every 1000uLs of cells used. In the single drug setup, 100uL of 2x drug diluted in media from a 1000x DMSO-based working dilution is added to a well. In the two and three drug set up, 50uL of 4x drug is added. The dmso-based working concentration for the two drug setup is 2000x while for the three drug setup is 4000x. The final outcome of each setup is a cell concentration of 0.25 million per mL, 1x final concentration of the utilized drugs, 0.1% (vol/vol) DMSO in media, and a final volume of 200uL. Delivery of at least 50uL ensures precise volumes and simple mixing by diffusion.