Jun 13, 2023

Public workspaceAnimal housing and ethical concerns

DOI
dx.doi.org/10.17504/protocols.io.14egn2oepg5d/v1
  • 1Mitochondria and Inflammation in Neurodegenerative Diseases, DZNE, Tübingen-Germany;
  • 2Hertie Institute for Clinical Brain Research, University of Tübingen;
  • 3Neuropharmacology Laboratory, Oasi Research Institute-IRCCS, Troina, Italy;
  • 4Biomedical and Biotechnological Sciences, Pharmacology Section, University of Catania-Italy
carmela giachino
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DOI: dx.doi.org/10.17504/protocols.io.14egn2oepg5d/v1
Protocol Citation: michela.deleidi, Bianca Marchetti, Federico Bertoli, Carmela Giachino 2023. Animal housing and ethical concerns. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn2oepg5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 31, 2023
Last Modified: June 13, 2023
Protocol Integer ID: 79799
Keywords: Animal housing, ethical concerns
Abstract
This protocol details animal housing and ethical concerns.
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Animal housing and ethical concerns
Animal housing and ethical concerns
Males C57BL/6J wild type (WT) and C57BL/6J LRRK2*G2019S 2AMjff/J transgenic (TG-G2019S) (stock 018785) mice purchased from Jackson Laboratory (Bar Harbor, ME, USA) at 8-12 weeks of age were housed at the animal facilities unit (C.A.Pi.R. Via Santa Sofia 97, Catania 13/2017-UT) and maintained on a 12:12-h light/dark cycle with ad libitum food and water.
The animals were given 3 weeks to acclimate to the housing conditions prior to study commencement.
Note
The research project (authorization n. 345/2019-PR released by the Italian Ministry of Health 06-05-2019) and all experimental procedures were approved by the University Institutional Animal Experimentation and Ethical Committee (OPBA, University of Catania) and performed in accordance with NIH Guide for the Care and Use of Laboratory Animals. Experiments were carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki) for animal experiments.