REAGENT or RESOURCE | SOURCE | IDENTIFIER | |
Cell line | |||
NGN2 inducible embryonic stem cells | See protocol dx.doi.org/10.17504/protocols.io.br9em93e | CVCL_9773 (modified from this source line) | |
Chemicals | |||
Dulbecco’s MEM (DMEM), F12 | |||
Phosphate Buffered Saline 1X | Corning | 21-031-CV | |
16% Paraformaldehyde, Electron-Microscopy Grade | Electron Microscopy Science | 15710 | |
Triton-X | Sigma | T8787 | |
Click-iT‱ Plus TUNEL Assay Kits for In Situ Apoptosis Detection , Alexa Fluor 488 | Thermo Fisher Scientific | C10617 | |
DAPI | Thermo Fisher Scientific | D1306 | |
E8 components | See protocol dx.doi.org/10.17504/protocols.io.bsacnaaw | ||
ND1 and ND2 components | See protocol dx.doi.org/10.17504/protocols.io.br9em93e | ||
Software | |||
Nikon Imaging Software Elements | 5.21.3 (Build 1489) | https://cellprofiler.org/ SCR_014329 | |
Cell Profiler | CellProfiler v4.0.6 | https://cellprofiler.org/ SCR_007358 | |
Fiji | ImageJ V.2.0.0 | https://imagej.net/software/fiji/ SCR_002285 | |
Hardware | |||
Thermo Neon‱ Transfection System | Thermo Fisher Scientific | MPK5000 | |
Yokogawa CSU-X1 spinning disk confocal on a Nikon Ti-E inverted microscope |