Untreated sewage from a domestic sewage treatment plant
Untreated sewage from hospital sewage treatment plant
Chicken fecal samples from the poultry farm
Sample collection bottles
Sterile 250 mL Filtration Unit
Sterile 250 ml conical flask
0.2 µm filtration membrane
Sterile 250 ml reagent bottle
Sterile latex hand gloves
Sterile Tryptone Soy Broth (TSB)
Frozen/streaked culture of MRSA
Sterile 50 mL centrifuge tube
Sterile micropipette tips
Mixed sample filtrate (from Step 1)
Sterile 2X TSB (HiMedia, India)
Sterile 50 mL glass conical flask (Borosil, India)
Overnight grown pure culture of MRSA (from Step 2)
Sterile micropipette tips
Sterile Soft agar (TSB + 0.6% Agar)
Overnight grown pure culture of MRSA (from Step 2)
Sterile 5 ml / 15 ml centrifuge tubes
Manual colony counter (optional)
Sterile micropipette tips