Jan 11, 2024
Akoya Biosciences PhenoCycler Fusion (formerly CODEX) User Guide
- 1Department of Medicine, Washington University in St. Louis
Protocol Citation: Andrew Houston 2024. Akoya Biosciences PhenoCycler Fusion (formerly CODEX) User Guide. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9p4j1g3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 11, 2024
Last Modified: January 11, 2024
Protocol Integer ID: 93394
Abstract
Link to PhenoCycler User Guide to allow for multi-plex spatial proteomic detection on fresh frozen and FFPE tissue sections
Staining/slide prep protocol can be found here:
PhenoCycler-Fusion User Guide_2.1.0.pdf15.3MB FFPE tissue staining pgs. 45-56
Reporter plate preparation pgs. 70-75
Imaging protocol can be found here:
PhenoImager Fusion User Guide_V1_1 Flow Cell_ 2.1.0.pdf6.2MB Flow cell assembly pgs. 29-39
Instrument set-up for PhenoCycler scan pgs. 57-71