Mar 12, 2023

Public workspaceAi14 Genotyping 

DOI
dx.doi.org/10.17504/protocols.io.81wgbywy1vpk/v1
  • 1BYU
Ronald.zegarra
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DOI: dx.doi.org/10.17504/protocols.io.81wgbywy1vpk/v1
Protocol Citation: Ronald.zegarra 2023. Ai14 Genotyping . protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbywy1vpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: March 09, 2023
Last Modified: March 12, 2023
Protocol Integer ID: 78457
Abstract
genotyping
Thaw reagent on ice forDuration00:10:00 minutes

10m
PrepareSampleMaster Mix :

Required
13.1 uL milliQ
Required
2 uL 10x PCR Buffer
Required
0.5 Advance 2 Polymerase Mix
Required
0.4 uL 10 mM dNTP
Required
1 uL Ai14 WT Forward
Required
1 uL Ai14 WT Reverse
Required
1 uL Ai14 Mutant Forward
Required
1 uL Ai14 Mutan Reverse

Pipette Amount19 µL of SampleMaster Mix into 0.2 mL Tube


Lightly Vortex DNA sample for Duration00:00:30

30s
Add Amount1 µL of DNA to each tube

Select "Ai14" PCR protocol on the thermocycler
Run 15 uL of PCR samples and a DNA ladder on a
Required
200 uL 1x TAE
Required
4 mg of agarose
at 120 V for Duration01:00:00 or until the dye is somewhat in the middle of the gel.

1h