For example, we have observed that the closer you are to the bone in the metaphysis, the more adipocytes you will have. Conversely, in the center of the metaphysis, you will have fewer adipocytes. Indeed, the biggest part of the heterogeneous organization of your BM depends on the proximity to the bone and different areas (metaphysis, diaphysis, or epiphysis). To avoid this problem, we can cut transversely to keep the same proportion of endosteal and non-endosteal niches between your different conditions. Obviously for transverse cuts, you need to compare the same bone area: metaphysis, diaphysis, or epiphysis.