Feb 12, 2019

Public workspaceADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes V.1

  • 1University of Alberta
  • Human Cell Atlas Method Development Community
  • CIRTNR2FIC
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External link: www.isletcore.ca
Collection CitationJames Lyon, Aliya F Spigelman, Patrick E Macdonald, Jocelyn E Manning Fox 2019. ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes. protocols.io https://dx.doi.org/10.17504/protocols.io.x3mfqk6
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 12, 2019
Last Modified: February 12, 2019
Collection Integer ID: 20301
Keywords: Human Islets, Islets of Langerhans, Cryopreservation, IsletCore
Abstract
This collection of protocols describe the processes used by the Alberta Diabetes Institute IsletCore for the isolation of human pancreatic islets of Langerhans for research. In addition, protocols related to quality control and the cryopreservation of human islets are included.

The ADI IsletCore program is described in full here:
Lyon J, Manning Fox JE, Spigelman AF, Kim R, Smith N, O'Gorman D, Kin T, Shapiro AMJ, Rajotte RV, MacDonald PE (2016) Research-focused isolation of human islets from donors with and without diabetes at the Alberta Diabetes Institute IsletCore. Endocrinology, 157(2): 560-569.
Files
Protocol
Icon representing the file Isolation of Human Pancreatic Islets of Langerhans for Research
Name
Isolation of Human Pancreatic Islets of Langerhans for Research
Version 2
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Human Islet Isolation Media Preparation
Name
Human Islet Isolation Media Preparation
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file IsletCore Equipment Setup for Human Islet Isolation
Name
IsletCore Equipment Setup for Human Islet Isolation
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Human Pancreas Biopsy Collection, ex vivo
Name
Human Pancreas Biopsy Collection, ex vivo
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Human Islet Purification - COBE setup
Name
Human Islet Purification - COBE setup
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Human Islet Quantification and Purity Assessment
Name
Human Islet Quantification and Purity Assessment
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Sampling of Human Islets for Quality Control Purposes
Name
Sampling of Human Islets for Quality Control Purposes
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Human Islet Cryopreservation
Name
Human Islet Cryopreservation
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Thawing Cryopreserved Human Islets
Name
Thawing Cryopreserved Human Islets
Version 1
, University of Alberta
Jocelyn E Manning FoxUniversity of Alberta
Protocol
Icon representing the file Static Glucose-stimulated Insulin Secretion (GSIS) Protocol - Human Islets
Name
Static Glucose-stimulated Insulin Secretion (GSIS) Protocol - Human Islets
Version 3
, University of Alberta
Aliya F SpigelmanUniversity of Alberta