Collection Citation: James Lyon, Aliya F Spigelman, Patrick E Macdonald, Jocelyn E Manning Fox 2019. ADI IsletCore Protocols for the Isolation, Assessment and Cryopreservation of Human Pancreatic Islets of Langerhans for Research Purposes. protocols.io https://dx.doi.org/10.17504/protocols.io.x3mfqk6
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 12, 2019
Last Modified: February 12, 2019
Collection Integer ID: 20301
Keywords: Human Islets, Islets of Langerhans, Cryopreservation, IsletCore
Abstract
This collection of protocols describe the processes used by the Alberta Diabetes Institute IsletCore for the isolation of human pancreatic islets of Langerhans for research. In addition, protocols related to quality control and the cryopreservation of human islets are included.
The ADI IsletCore program is described in full here:
Lyon J, Manning Fox JE, Spigelman AF, Kim R, Smith N, O'Gorman D, Kin T, Shapiro AMJ, Rajotte RV, MacDonald PE (2016) Research-focused isolation of human islets from donors with and without diabetes at the Alberta Diabetes Institute IsletCore. Endocrinology, 157(2): 560-569.
