Jun 22, 2022

Public workspaceAddition of Antibiotic Supplement to Media 

This protocol is a draft, published without a DOI.
  • 1Fulbright Program
Mariam Alkattan
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Protocol Citation: Mariam Alkattan 2022. Addition of Antibiotic Supplement to Media . protocols.io https://protocols.io/view/addition-of-antibiotic-supplement-to-media-cbsksncw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 22, 2022
Last Modified: June 22, 2022
Protocol Integer ID: 65068
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Abstract
The following protocol is for the addition of antibiotic stock solution to molten media.
Materials
Equipment
  • thermometer; laser infrared (preferred) or stem
  • adjustable pipette
  • pipette tips


Consumables
  • antibiotic stock solution

Retrieve molten media from autoclave using heat resistant gloves. Place media in laminar flow hood if one is available. If laminar flow hood is not available, place media next to open flame on lab bench.
Safety information
To further protect from burn injuries, keep heat resistance gloves on and use box or bin to transport hot media if you plan to move media more than a few feet from autoclave.

Wait for media to cool toTemperature50 °C . Measure temperature using laser infrared thermometer or a stem thermometer. If using stem thermometer, sterile using 70% ethanol and allow to dry right before use.

When media reaches Temperature50 °C , use an adjustable pipette to add appropriate volume of antibiotic stock solution to molten media.

Gently swill media in bottle to mix antibiotic.
Note
If media is being used to pour plates, pour plates immediately after last step.