If running the experiment beyond step 5, prepare the buffers:
We recommend making all the buffers fresh on the day.
Prepare 2x Salts and Tris (ST) buffer. For 5 mL, mix:
Mix well and store on ice until use.
Prepare 1% CHAPS. For 10 mL, mix:
10 ml sterile H2O
0.1g CHAPS hydrate
Mix well and store on ice until use.
5.0 mL 2X ST
4.9 mL 1% CHAPS
100 μL 1% BSA
40 μL SUPERase IN
40 μL RNasein Plus
1 cOmplete tablet
Mix well and store on ice until use.
Prepare PBS + 1% BSA. For 20 mL, mix:
Mix well and store on ice until use.