Oct 07, 2023
Activation of Simvastatin
- 1University of California, Irvine
Protocol Citation: Dennis Juarez, David Fruman 2023. Activation of Simvastatin. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkwd5xl5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 21, 2022
Last Modified: October 07, 2023
Protocol Integer ID: 74349
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Abstract
Activation of simvastatin lactone to active simvastatin acid by hydrolysis of lactone ring with NaOH.
Materials
Simvastatin was obtained from Cayman Chemical Company
Preparation
Preparation
For every 8.4 mg of simvastatin sodium salt, dissolve in 0.2 mL of 100% ethanol.
Add 30 uL of 1N NaOH for every 8.4 mg of simvastatin sodium salt.
Heat the solution for at 50 °C for 2 hours.
Neutralize the solution to pH 7.2 with 1N HCl.
Bring to a volume of 1 mL per 8.4 mg of simvastatin dissolved in Step 1 with distilled H2O.
Dilute 1:1 with DMSO to yield a 10mM solution with 50% DMSO, and 10% EtOH in H2O.
Store at -80 °C