Mar 20, 2023
Acid Wash Protocol (Alegado Lab)
- 1Alegado Lab
Protocol Citation: Ashleyolguin 2023. Acid Wash Protocol (Alegado Lab). protocols.io https://dx.doi.org/10.17504/protocols.io.14egn213qg5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 20, 2023
Last Modified: March 20, 2023
Protocol Integer ID: 79075
Abstract
This protocol guides lab members through the acid wash process
Pre-requisites (BEFORE proceeding to acid washing)
Pre-requisites (BEFORE proceeding to acid washing)
Check acid solution levels
--> Note: If it's only 1/3 full, make new solution, you will use almost all of the current stock.
Check to see if Alegado Acid Wash Bin is free or if it is being used
Gather bottles to be washed and take over to acid washing lab
Acid Washing
Acid Washing
Fill bin with 10% Hydrochloric acid solution about ½ to 2/3 full and submerge bottles in bin.
Fill bottles with acid solution, cap and place in bin.
Cover bin with lid.
--> Note: insure cover is secured.
Let sit for minimal of 2 hours.
--> Note: place bin on Acid wash rack.
Rinse with miliQ water
Dry on designated drying surface
Empty bin and rinse with miliQ water.
Dry
Neutralization of Acid
Neutralization of Acid
Plug the drain and test the seal using water
Use acid rinse until the sink fills 3/4 maximum
Add sodium bicarbonate
Test the pH using a test strip
- If the measured pH is between 5.5 and 8.5, unplug the drain and discharge. Repeat the procedure for any remaining acid rinsing.
- If the pH is below 5.5, add more sodium bicarbonate
- If the pH is above 8.5, add more acid.
Always test the pH before discharging !