License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 15, 2022
Last Modified: April 19, 2022
Protocol Integer ID: 60819
Abstract
aa-Onepot-seq follows protocol below:
Cells and beads preparation and incubation
Transfer and Scatter Cell-bead complexes
Cell lysis and beads isolation
cDNA synthesis
cDNA library amplification
NGS library preparation
Guidelines
This protocol is modified based on Onepot-seq protocol.