Mar 07, 2024

Public workspaceA RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols

DOI
dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
  • 1University of California, Berkeley
Dan Tudorica
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DOI: dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
Collection Citation: Dan Tudorica 2024. A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 11, 2024
Last Modified: March 07, 2024
Collection Integer ID: 93365
Abstract
Methods used in this paper.
Files
Protocol
Icon representing the file HaloTag autophagy flux assay
Name
HaloTag autophagy flux assay
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Pulldowns 
Name
Pulldowns 
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Stable cell line generation via retrovirus
Name
Stable cell line generation via retrovirus
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Plasmid construction
Name
Plasmid construction
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
Name
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Immunofluorescent Imaging and Analysis
Name
Immunofluorescent Imaging and Analysis
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file WIPI2d puncta formation assay
Name
WIPI2d puncta formation assay
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Homology Modelling of Pacer RH domain in SWISS-MODEL
Name
Homology Modelling of Pacer RH domain in SWISS-MODEL
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Expression and Purification of RH proteins via MBP affinity tag
Name
Expression and Purification of RH proteins via MBP affinity tag
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Confocal-based bead-binding
Name
Confocal-based bead-binding
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Rab7 Phosphorylation reaction
Name
Rab7 Phosphorylation reaction
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Tissue culture of HeLa cells
Name
Tissue culture of HeLa cells
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Icon representing the file Western blotting for Rubicon and Pacer expression
Name
Western blotting for Rubicon and Pacer expression
Version 1
, Hurley Lab, QB3, UC Berkeley
Dan TudoricaHurley Lab, QB3, UC Berkeley