Mar 07, 2024

Public workspaceA RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols

DOI
dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
  • 1University of California, Berkeley
Dan Tudorica
Open access
DOI: dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
Collection Citation: Dan Tudorica 2024. A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy - protocols. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qy7jvo5/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 11, 2024
Last Modified: March 07, 2024
Collection Integer ID: 93365
Abstract
Methods used in this paper.
Files
Protocol
HaloTag autophagy flux assay
Name
HaloTag autophagy flux assay
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Pulldowns 
Name
Pulldowns 
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Stable cell line generation via retrovirus
Name
Stable cell line generation via retrovirus
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Plasmid construction
Name
Plasmid construction
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
Name
Isothermal Titration Calorimetry of the Rubicon RH domain and Rab7
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Immunofluorescent Imaging and Analysis
Name
Immunofluorescent Imaging and Analysis
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
WIPI2d puncta formation assay
Name
WIPI2d puncta formation assay
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Homology Modelling of Pacer RH domain in SWISS-MODEL
Name
Homology Modelling of Pacer RH domain in SWISS-MODEL
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Expression and Purification of RH proteins via MBP affinity tag
Name
Expression and Purification of RH proteins via MBP affinity tag
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Confocal-based bead-binding
Name
Confocal-based bead-binding
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Rab7 Phosphorylation reaction
Name
Rab7 Phosphorylation reaction
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Tissue culture of HeLa cells
Name
Tissue culture of HeLa cells
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley
Protocol
Western blotting for Rubicon and Pacer expression
Name
Western blotting for Rubicon and Pacer expression
Version 1
Dan Tudorica
Dan TudoricaHurley Lab, QB3, UC Berkeley