Jul 08, 2015
YES Rich Medium
- Alan J. Cone1
- 1Wright State University
- Ju Lab
Protocol Citation: Alan J. Cone 2015. YES Rich Medium. protocols.io https://dx.doi.org/10.17504/protocols.io.dbf2jm
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 08, 2015
Last Modified: November 10, 2017
Protocol Integer ID: 1095
Abstract
Similar to YPD Medium for budding yeast, YES Medium is the rich growth medium utilized for fission yeast.
Materials
MATERIALS
Yeast ExtractCatalog #Y1625
AdenineCatalog #A8626
UracilCatalog #U0750
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-LeucineCatalog #L8000
L-LysineCatalog #L5626
L-ArginineCatalog #A5006
Difco Bacto AgarCarolinaCatalog #156783B
STEP MATERIALS
Yeast ExtractCatalog #Y1625
GlucoseP212121Catalog #Glucose
AdenineCatalog #A8626
UracilCatalog #U0750
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-LeucineCatalog #L8000
L-LysineCatalog #L5626
L-ArginineCatalog #A5006
Difco Bacto AgarCarolinaCatalog #156783B
Yeast ExtractCatalog #Y1625
GlucoseP212121Catalog #Glucose
AdenineCatalog #A8626
UracilCatalog #U0750
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-LeucineCatalog #L8000
L-LysineCatalog #L5626
L-ArginineCatalog #A5006
Difco Bacto AgarCarolinaCatalog #156783B
Protocol materials
UracilCatalog #U0750
L-LysineCatalog #L5626
L-ArginineCatalog #A5006
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-LysineCatalog #L5626
Difco Bacto AgarCarolinaCatalog #156783B
GlucoseP212121Catalog #Glucose
L-ArginineCatalog #A5006
Difco Bacto AgarCarolinaCatalog #156783B
L-Histidine monohydrochloride monohydrateCatalog #H8125
Difco Bacto AgarCarolinaCatalog #156783B
L-LeucineCatalog #L8000
L-LysineCatalog #L5626
AdenineCatalog #A8626
L-LeucineCatalog #L8000
AdenineCatalog #A8626
UracilCatalog #U0750
L-ArginineCatalog #A5006
Yeast ExtractCatalog #Y1625
GlucoseP212121Catalog #Glucose
Yeast ExtractCatalog #Y1625
Yeast ExtractCatalog #Y1625
AdenineCatalog #A8626
UracilCatalog #U0750
L-LeucineCatalog #L8000
Yeast ExtractCatalog #Y1625
GlucoseP212121Catalog #Glucose
AdenineCatalog #A8626
UracilCatalog #U0750
L-Histidine monohydrochloride monohydrateCatalog #H8125
L-LeucineCatalog #L8000
L-LysineCatalog #L5626
L-ArginineCatalog #A5006
Difco Bacto AgarCarolinaCatalog #156783B
Mixing
Mixing
Add 5.0 g Yeast Extract to a 1 Liter Erlenmeyer Flask.
5 g
Yeast ExtractCatalog #Y1625
Add 30 g Glucose or Dextrose to the flask.
30 g
GlucoseP212121Catalog #Glucose
Add 0.25 g Adenine to the flask.
0 g
AdenineCatalog #A8626
Add 0.25 g Uracil to the flask.
0 g
UracilCatalog #U0750
Add 0.25 g Histidine to the flask.
0 g
L-Histidine monohydrochloride monohydrateCatalog #H8125
Add 0.25 Leucine to the flask.
0 g
L-LeucineCatalog #L8000
Add 0.25 g Lysine to the flask.
0 g
L-LysineCatalog #L5626
Add 1 g Arginine to the flask.
1 g
L-ArginineCatalog #A5006
Optional: For solid medium add 20 g Difco Bacto Agar to the flask.
20 g
Difco Bacto AgarCarolinaCatalog #156783B
Fill the flask with water up to the 1 Liter mark on the flask.
1 L
Place a magnetic stir bar into the flask and place the flask on a magnetic stirrer to dissolve the powder into the water.
00:10:00
Remove the stir bar with a magnetic rod.
Autoclaving
Autoclaving
Place aluminum foil over the top of the flask and then autoclave on a liquid cycle for 20 minutes.
01:00:00
If solid medium, pour while still hot into plates and let them sit out to cool overnight on the lab bench.
If liquid medium, aliquot into smaller amounts and store at + 4 C.
Note
Solid medium must be poured while still hot, otherwise it can solidify inside the flask.
Note
Storing liquid medium in small aliquots helps prevent against widespread contamination through normal use.
Note
After solid medium-filled plates cool, store inverted at +4 C.