Aug 07, 2015
RIPA lysis buffer (strong)
- Vivian Liu
Protocol Citation: Vivian Liu 2015. RIPA lysis buffer (strong). protocols.io https://dx.doi.org/10.17504/protocols.io.dky4xv
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: August 07, 2015
Last Modified: March 27, 2018
Protocol Integer ID: 1400
Guidelines
| Tris-HCl pH7.4 | 50 mM | |
| NaCl | 150 mM | |
| Sodium Deoxycholate | 1 % | |
| SDS | 0.10 % | |
| Triton-X100 | 1 % | |
| EDTA | 0.5 mM | |
| Na3VO4 | 1 mM | |
| NaF | 50 mM |
Sterilize by passing through 0.22 µm filter. Store at 4 °C. Add protease inhibitor cocktails just before using.
Materials
MATERIALS
Sodium Dodecyl SulfateP212121
Sodium deoxycholateP212121
TrisP212121
Sodium fluorideP212121
Sodium orthovanadate
Sodium chlorideP212121
Triton-X100