Feb 09, 2016
Pellet resuspension
- André M. Comeau and Rachel T. Noble1
- 1Manual of Aquatic Viral Ecology
- VERVE Net
External link: http://www.aslo.org/books/mave/MAVE_019.pdf
Protocol Citation: André M. Comeau and Rachel T. Noble 2016. Pellet resuspension. protocols.io https://dx.doi.org/10.17504/protocols.io.dzt76m
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: October 15, 2015
Last Modified: November 10, 2017
Protocol Integer ID: 1811
Abstract
Pipette off 1.5 mL using a P1000.
Switch to a P100 and gently remove nearly all of the supernatant.
Note
Remainder usually totals ~10 µL.
Add 40 µL water or seawater medium to bring the volume to 50 µL.
Note
Remember freshwater versus seawater choice for viral isolate.
Gently vortex the tubes to disrupt the pellets and place them at 4°C overnight to soften.
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