Jul 28, 2016

Public workspaceMurashige and Skoog (MS) medium

DOI
dx.doi.org/10.17504/protocols.io.fdjbi4n
  • Steven Burgess1
  • 1University of Cambridge
  • OpenPlant Project
  • GEGC lab UIUC
Steven J Burgess
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.fdjbi4n
External link: https://en.wikipedia.org/wiki/Murashige_and_Skoog_medium
Protocol Citation: Steven Burgess 2016. Murashige and Skoog (MS) medium. protocols.io https://dx.doi.org/10.17504/protocols.io.fdjbi4n
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 22, 2016
Last Modified: March 28, 2018
Protocol Integer ID: 3211
Abstract
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MSO was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/l sucrose. Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in laboratorium.[1]
Source:
http://cshprotocols.cshlp.org/content/2010/2/pdb.rec12142.full?text_only=true
Guidelines
Derivatives of MS medium can be made through the addition of sucrose, both 1% (w/v) and 2% (w/v) are commonly used. 
Murashige and Skoog basal medium
Amount4 g
dH2O
Amount800 mL
2 N Potassium hydroxide
KOH
Note
Adjust pH to 5.7
dH2O
Note
Add up to 1L