Jul 20, 2015

Public workspaceEDTA disodium salt dihydrate

DOI
dx.doi.org/10.17504/protocols.io.dbm2k5
  • James Thornton1
  • 1Matthew Sullivan Lab, University of Arizona/Ohio State University
  • VERVE Net
  • Sullivan Lab
Bonnie Poulos
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DOI: dx.doi.org/10.17504/protocols.io.dbm2k5
Protocol Citation: James Thornton 2015. EDTA disodium salt dihydrate. protocols.io https://dx.doi.org/10.17504/protocols.io.dbm2k5
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 08, 2015
Last Modified: March 23, 2018
Protocol Integer ID: 1101
Abstract
For use in DNase I Treatment protocol for DNase inactivation.
Guidelines
Note: EDTA tetrasodium salt dihydrate is somewhat more soluble and can be made more easily to 1.5M. FW is slightly more so weigh out 3.80 g and follow directions above.
Weigh out 3.72 g EDTA and place in 5 ml molecular biology grade water.
Add a few NaOH pellets to get to pH 9 and dissolve EDTA (can warm to 45°C with stirring to aid in dissolution).
Measure volume and determine molarity: 1M = 3.72g/10 ml, therefore, measured volume/10 ml = final molarity.