Jan 08, 2016

Public workspaceAscorbic-EDTA Buffer

DOI
dx.doi.org/10.17504/protocols.io.c8xzxm
  • B.R. Cunningham1,
  • J.R. Brum1,
  • S.M. Schwenck1,
  • M.B. Sullivan1,
  • S.G. John1
  • 1Matthew Sullivan Lab, University of Arizona/Ohio State University
  • VERVE Net
  • Sullivan Lab
Verve Team
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DOI: dx.doi.org/10.17504/protocols.io.c8xzxm
Protocol Citation: B.R. Cunningham, J.R. Brum, S.M. Schwenck, M.B. Sullivan, S.G. John 2016. Ascorbic-EDTA Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.c8xzxm
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: July 01, 2015
Last Modified: January 18, 2018
Protocol Integer ID: 1015
Abstract
Guidelines
Note: An alternative Ascorbate-EDTA Buffer can be made with MgCl2 and Na2EDTA if Mg2EDTA is unavailable:
- Combine equal parts of 0.125 M Tris-base, 0.1 M Na2-EDTA dihydrate, 0.2 M MgCl2 hexahydrate, and 0.2 M ascorbic acid, adjust with 10 N NaOH to reach a pH of 6-7.
- 0.125 M Tris-base prepared by dissolving 0.151 g Tris-base in 10 mL ultrapure water
- 0.1 M Na2-EDTA dehydrate prepared by dissolving 0.372 g Na2- EDTA dehydrate in 10 mL ultrapure water
- 0.2 M MgCl2 hexahydrate prepared by dissolving 0.407 g MgCl2 hexahydrate in 10 mL ultrapure water
- 0.2 M ascorbic acid prepared by dissolving 0.352 g ascorbic acid in 10 mL ultrapure water
Combine equal parts of 0.4 M Mg2EDTA and 0.8 M ascorbic acid, adjust with 10 N NaOH to reach a pH of 6-7.
Prepare fresh within 48 hours of use