Worksheet 603.A.2 HTC_Whole_or_Partial_Lung_Processing
Biosafe surface and environment for manipulation of lung
Grossing Station, Biosafety Cabinet or Fume Hood
Gloves, face protection and clothing consistent with blood and body fluid precautions
Biohazards Disposal Bag
Red Sharps Container
Tissue mega-cassettes (optional) and uni-cassettes
Scalpels and Trimming Blades with Handles; Forceps; Other Dissection Equip
Labeling pencil
10% neutral buffered formalin – at least one liter, with more on hand if needed for larger lungs
1xPhosphate Buffered Sodium
30% Ethanol; 50% Ethanol and 70% Ethanol. All solutions are made with Diethylpyrocarbonate (DEPC) treated water or directly treated with DEPC to minimize RNA degradation
Small and Medium Gauze Pads – multi-pack
Standard Balance and Weigh Boats: Medium and Large
Airway Cannulas – Selection of sizes
(i) 16 and 18 gauge angiocatheters (needles removed and discarded)
(ii) tracheal cannulas 2.0-4.0 OD
(iii) endotracheal tubes 2.5 – 8 mm OD; tubes >/= 4.0 cuffed
14. IV extension set tubing with clamp
15. 20 ml syringe barrels for fixative
16. Needle free suture material