Apr 10, 2024

Public workspace3D printed stereotax multimodal imaging compatibility assessment

DOI
dx.doi.org/10.17504/protocols.io.j8nlk85edl5r/v1
  • 1University of Pittsburgh
Lucy Liang
Open access
DOI: dx.doi.org/10.17504/protocols.io.j8nlk85edl5r/v1
Protocol Citation: Isabela Zimmermann Rollin, Lucy Liang, David J Schaeffer 2024. 3D printed stereotax multimodal imaging compatibility assessment. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk85edl5r/v1
Manuscript citation:
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 26, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 97401
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: ASAP-020519
Abstract
PET/CT and MRI for multimodal image compatibility.

This protocol is supplementary to the manuscript:
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767
Guidelines
The FDG doses are for proof of concept, and even smaller doses of a radioligand may suffice for practical use, depending on exposure and technical considerations.
Materials
Imaging Equipment
  • Small preclinical PET/CT scanner (Si78; Bruker BioSpin GmbH, Ettlingen, Germany)
  • Paravision-360 software package (version 3.2; Bruker BioSpin Corp, Billerica, MA, RRID: SCR_001964)

Materials
PET/CT and MRI for multimodal image compatibility
PET/CT and MRI for multimodal image compatibility
Preparation of eye bars and ear bars: With a syringe, fill the ear and eye bars with a small dose (0.05 MBq ear bar, 1.54 MBq eye bar) of [18F] fluorodeoxyglucose (FDG) and seal the chamber with a MRI-compatible nylon screw. Note that these doses are for proof of concept, and even smaller doses of a radioligand may suffice for practical use, depending on exposure and technical considerations.

image.png


PET Imaging: Place the ear and eye bars in a small preclinical PET/CT scanner (here we used the Si78; Bruker BioSpin GmbH, Ettlingen, Germany) and use the Paravision-360 software package (version 3.2; Bruker BioSpin Corp, Billerica, MA, RRID: SCR_001964) for image acquisition.
CT Acquisition: Acquire a CT scan using the same PET/CT scanner equipped with a Low Dose 1 mm aluminum filter. Here we used a “step and shoot” method with a 0.6-degree gantry step for image acquisition.
Image Reconstruction: Reconstruct the CT images using the filtered back projection algorithm.
Protocol references
3D printable stereotaxic frame: https://github.com/SchaefferLab/Macaque-Stereotax
Liang, L., Zimmermann Rollin, I., Alikaya, A., Ho, J.C., Santini, T., Bostan, A.C., Schwerdt, H.N., Stauffer, W.R., Ibrahim, T.S., Pirondini, E., Schaeffer, D.J., 2024. An open-source MRI compatible frame for multimodal presurgical mapping in macaque and capuchin monkeys. BioRxiv https://doi.org/10.1101/2024.02.17.580767